High Throughput Proteomics Profiling

High throughput drug profiling

High throughput screening has significantly contributed to advances in drug discovery. The great increase in the number of samples screened has been accompanied by increases in costs and in the data required for the investigated compounds. High throughput profiling addresses the issues of compound selectivity and specificity. It combines conventional screening with data mining technologies to g...

High-throughput expression profiling techniques.

Great optimism exists that correlating genomics with function will lead to a better understanding of the detailed workings of the nervous system. The nearly complete sequencing of the genomes of several eukaryotic species and the invention of high-throughput expression profiling techniques now provide the means to rapidly investigate the molecular underpinnings of phenotypic change with reasona...

A streamlined approach to high-throughput proteomics.

Proteomics has rapidly become an important tool for life science research, allowing the integrated analysis of global protein expression from a single experiment. To accommodate the complexity and dynamic nature of any proteome, researchers must use a combination of disparate protein biochemistry techniques, often a highly involved and time-consuming process. Whilst highly sophisticated, indivi...

High-throughput Image Analysis for Proteomics

High-throughput phosphotyrosine profiling using SH2 domains.

Protein tyrosine phosphorylation controls many aspects of signaling in multicellular organisms. One of the major consequences of tyrosine phosphorylation is the creation of binding sites for proteins containing Src homology 2 (SH2) domains. To profile the global tyrosine phosphorylation state of the cell, we have developed proteomic binding assays encompassing nearly the full complement of huma...